Alveolar bone is an important part of the dynamic tooth-bone complex. In the case of mouse first molar, the alveolar bone becomes morphologically apparent at the embryonic (E) day 14. Gradually, the bone encapsulates the tooth germ and the next process, bone remodelling, starts to accommodate the growing tooth germ and create the final support during and after eruption. This worked aimed to search for candidates in osteogenic networks around the start of the alveolar bone formation. In the first part of research, stages E13, E14 and E15 were compared regarding expression of osteogenic genes using the Mouse Osteogenic PCRArray. Along with collagens, altered gene expression was observed in the case of Fgf3, Ctsk, Icam-1, Mmp9, Itga3 and Tuft1 where significant increase was demonstrated. Decrease or additional subtle changes were found also for other genes. The next step of investigation was focused on localisation of proteins corresponding to genes with increased expression within the mandibular/alveolar bone using immunohistochemistry. The findings were correlated with cell types and morphogenetic events in early alveolar bone formation.
Disclosure: The authors declared no competing interests. This research has been recently supported by the Grant Agency of the Czech Republic (14-37368G).