Constant remodelling of extracellular matrix (ECM) is a hallmark during physiological conditions, such as stem cell differentiation, embryogenesis and tissue repair. MMPs, MMP-inhibitors (TIMPs and RECK) and MMP-inducer (EMMPRIN) play a key role in these processes, being part of cell proteolytic secretome. Transforming Growth Factor Beta superfamily members, such as TGF-β and BMPs, are responsible for bone, tooth and cartilage formation and modulate the expression of molecules involved on matrix turnover. In this way, we evaluated gene expression of MMPs, TIMPs, RECK and EMMPRIN during induction of osteogenic/odontogenic and chondrogenic differentiation from DPSCs in vitro by qPCR. DPSCs were isolated from extracted human third molars and grown in clonogenic medium (α-MEM medium +10% FBS +100 μM ascorbate) and differentiation induction in presence of osteogenic medium (10 mM β-glycerophosphate, 1 μM dexamethasone and 100 μM ascorbate), odontogenic medium (10 mM β-glycerophosphate, 1 μM dexamethasone and 100 μM ascorbate +50 ng/mL BMP-7) or chondrogenic medium (DMEM/F12+500 ng/mL insulin +1 μM dexamethasone +50 μM ascorbate +10 ng/mL TGF-β1+1mM sodium pyruvate) for 35-days. For each differentiation, a differential gene expression pattern was observed and our results suggest that both TGF-β1 and BMP-7 may regulate MMPs, their inhibitors and inducer gene expression during osteogenic/odontogenic and chondrogenic differentiation in vitro from DPSCs.
Disclosure: The authors declared no competing interests. This work was supported by the São Paulo Research Foundation (FAPESP) (grant number 2010/08918-9), LHSS FAPESP fellowship (grant number 2011/04342-8) and KBSP FAPESP Young Scientist fellowship (grant number 2011/00204-0).