Vitamin D increases osteoblastogenesis, by stimulation of the signalling pathway Wnt / b-catenin, and represses expression of inhibitors of this pathway. Recent studies have suggested that vitamin D may be associated with glycaemic control, vascular calcification and cardiovascular disease (CVD). This study aims to evaluate the relationship between circulating 25-Hydroxyvitamin D (25-OHD), glycaemic control and inhibitors of the WNT pathway (sclerostin and DKK1) in patients with CVD. We conducted a cross-sectional study with 120 subjects (45 patients with CVD and 74 controls). The inclusion criteria for CVD were: ischaemic cerebrovascular disease, coronary heart disease and / or peripheral arterial disease. Surrogate markers of CVD (carotid intima media thickness [IMT], carotid plaques and aortic calcifications) were evaluated. Serum levels of DKK1 sclerostin and inhibitors were measured by ELISA immunoassay. Serum 25-OHD levels were significantly lower in CVD group (16.4±8.1 ng / ml vs 20.7±11.0 ng / ml, p=0.025) patients with carotid plaque (14.0±6.6 ng / ml vs 20.6±10.9 ng / ml, p=0.001) and vascular calcification (15.2±6.6 ng / ml vs 20.1±11.7 ng / ml, p=0.012). Serum 25-OHD showed a negative correlation with fasting glucose (r=-0,254, p=0.004), HbA1c (r=-0,186, p=0.037), PTH (r=-0,205, p=0.028), sclerostin (r=-0,191, p=0.032) and IMT (r=-0,178, p=0.037). The multivariate regression model shows sclerostin as the only variable independently associated with serum 25-OHD (β=-0201; CI =-0220 to 0.002, p=0.047). Circulating 25-OHD could modulate Wnt signalling pathway by regulating the expression of sclerostin in patients with CVD. Supplementation with vitamin D could improve the health status in this population.
Disclosure: The authors declared no competing interests. The study was support by grants from the Consejería de Salud y Bienestar Social. Junta de Andalucía (PI-0514-2012) and by the Red Temática de Investigación Cooperativa en Envejecimiento y Fragilidad (RETICEF) of the Instituto Carlos II, Spanish Ministry of Economy and Competitiveness (RD12/0043/0014).