Periodontitis is a chronic inflammatory disease and induces periodontal tissue destruction. Periodontal ligament cell-based treatment is considered as one of the most promising methods in periodontal tissue regeneration. This study examined the regenerative effects and anti-destructive effects of glabridin on periodontal ligament fibroblasts (PDLFs). We assessed the regenerative effects of glabridin on PDLFs by measuring the expression of alkaline phosphatase, type I collagen, osteocalcin, Runx2 and osteoprotegerin using quantitative real-time PCR. We also determined the anti-destructive effects of glabridin on PDLFs under inflammatory conditions by examining the expression of proteolytic enzymes, including matrix metalloproteinase (MMP)-1, MMP-2 and MMP-8 using quantitative real-time PCR. Additionally, we evaluated the effects of glabridin on inflammatory mediators by measuring the secretion of interleukin -1β (IL-1β), tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and nitric oxide (NO) on RAW264.7 cells. Glabridin increased significantly alkaline phosphatase, Runx2 and osteoprotegerin mRNA expression. Glabridin suppressed the expression of MMP-1 and MMP-8 in the PDLFs. In addition, glabridin had no effect on viability of the RAW264.7 cells and decreased the release of LPS-induced IL-1β, TNF-α, IL-6 and NO in RAW264.7 cells. These findings suggest that glabridin can stimulate the osteogenic differentiation and alleviates the tissue-destructive processes that occur during periodontal inflammation.
Disclosure: The authors declared no competing interests. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (#2010-0023679).