ECTS Abstracts (2015) 1 P36

Bone loss comparing two experimental protocols that induce osteopenia

Melise J P Ueno, Ronaldo A Medeiros & Mario J Q Louzada

Unesp, Aracatuba, Sao Paulo, Brazil.

Many studies use different experimental protocols to induce osteopenia in rats, among which the most used are: tail suspension and ovariectomy. Our goal was to determine whether the different causes of osteopenia have the same effects on bone structure. For this study, 30 females Wistar rats of 19 weeks of age, were distributed into the groups: control group (GC), group suspension (GS) and ovariectomised group (OVX). The tail suspension was performed for 21 days to 24 weeks of age and the euthanasia of all animals was performed at 27 weeks of age. Bone mineral density (BMD) and biochemical markers of calcium, phosphorus, and alkaline phosphatase were analysed at the beginning and at the end of the experiment. The result showed that S femoral BMD decreased compared with the C (p=0.0350), while that at OVX decreased compared to the beginning of the experiment (p=0.0114), however was no difference with the control group (p=0.3940). By analysing the tibia, BMD in GS and OVX compared with the CG did not reach statistical difference, however BMD in SG decreased compared with the beginning of the experiment (p=0.0469). Biochemical assays showed that the plasma concentration of calcium was lower after the experiments in OVX and SG groups (p=0.0318 and p=0.0320, respectively), but both had no difference between the control. The phosphorus concentration of SG animals had a significant increase (p=0.0246), while the other groups maintained their concentration equilibrium. Finally, the activity of alkaline phosphatase in OVX rats was significantly higher compared to the beginning of the experiment (p=0.0427) whereas in SG there was a significant decrease (p=0.0078). We can conclude that in female rats, the severity of bone loss was greater in the tail suspension model.

Disclosure: The authors declared no competing interests.

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