Latest findings interpreted that not only degenerate, but also inflammatory are considered to involve in the development and progression of osteoarthritis (OA). Methylenetetrahydrofolate reductase (MTHFR) gene has been correlated with inflammation process. The function polymorphism C677T (rs1801133) mutation of MTHFR may lead to hyperhomocysteinemia so that enhances productions of several pro-inflammatory cytokines, such as IL6. In the light of these findings, we decided to survey if any relationship between MTHFR gene C677T mutation in Han Chinese population with knee osteoarthritis. We performed a case control study. 300 cases comprised patients with a radiologic scoring (Kellgren-Lawrence score) ≧2 and 500 controls with a radiologic scoring (K-L score)<2, both of them form healthy evaluation centres in Tri-Service General Hospital in Taipei, Taiwan. DNA was extracted from a peripheral blood sample, and the MTHFR gene C677T genotypes were determined by PCR-RFLP. Functional assay were performed using enzyme-linked immunosorbant assay (ELISA). Our results revealed that the MTHFR C677T polymorphism might increase the risk of OA (T allele vs. C allele: OR=1.78, 95% CI=1.01~3.14, P<0.05) after adjusted confounders, such as age, BMI, habitual physical activity. There were no significant in serum IL6 levels between various genotypes of C/T (P>0.05). These data suggest that MTHFR C677T polymorphism may be associated with osteoarthritis, MTHFR gene could be used as genetic markers in osteoarthritis in Han Chinese populations.
Disclosure: The authors declared no competing interests.