ECTS Abstracts (2015) 1 P251

Association of the Tissue Inhibitor of Metalloproteinases-3 (TIMP-3) Gene Polymorphism with Knee Osteoarthritis in Taiwan population

Shu-Pei Lee & Yu-Ching Chou


School of Public Health, National Defense Medical Center, Neihu, Taipei, Taiwan.


Osteoarthritis (OA) is characterised by the progressive failure of the extracellular cartilage matrix, which leads to the destruction of articular cartilage and pathologic changes in the joints. Studies have investigated the main cause of degrading cartilage is governed mainly by a balance between the degradation of matrix metalloproteinases (MMPs) and their tissue inhibitors of metalloproteinases (TIMPs). The aim of this study was to identify whether functional polymorphisms in the promoter region of the TIMP-3 G/A (rs715572) and MMP-3 5A/6A (rs3025058) genes were associated with knee osteoarthritis in Taiwan population. The present study is based on 210 Taiwanese OA patients as case group with a radiologic scoring (Kellgren-Lawrence score) ≧2 and 195 healthy subjects as control group with a radiologic scoring (K-L score) 0 and 1.Peripheral blood sample (5 ml) was collected from patient and healthy group. DNA was investigated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In our study, we found the association that TIMP-3 G/A (rs715572) gene polymorphism was significant difference between case and control group (P=0.032). Otherwise, no association of MMP-3 5A/6A (rs3025058) gene polymorphism was found between case and control group (P>0.05). These results have demonstrated a significant association between the polymorphism of TIMP-3 G/A gene with the susceptibility of OA in the Taiwanese population.

Disclosure: The authors declared no competing interests.

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