Osteoarthritis (OA) is a degenerative joint disorder resulting in destruction of articular cartilage, osteophyte formation, and subchondral bone sclerosis. Angiotensin converting enzyme (ACE) plays an prominent role to promote inflammation and has been particularly related to rheumatic and autoimmune diseases. It is a metalloenzyme converts angiotensin I to a potent vasoconstrictor angiotensin II and will inactivate bradykinin which is a vasodilator may implicates immune-related disease including osteoarthritis. The aim of the current study was to examine the influence of ACE gene insertion/deletion (I/D) variations on the risk of osteoarthritis. A total of 460 patients with OA and 400 healthy subjects, both of them from healthy evaluation centres were included in the study. The definition of OA is readers evaluated Kellgren-Lawrence grade ≥2 by using X-rays judgment. DNA was extracted from a peripheral blood sample and was amplified by PCR using I and D allele-specific primers. PCR products were assessed with via UV visualisation by being exposed to 1.5% agarose gels. A significant difference between patients and controls was found that in the frequencies of ACE I/D alleles, OA patients had a higher presence of D allele (OR=1.8, 95% CI=1.232.27, P<0.001) and the DD genotype (OR=2.03, 95% CI=1.243.03, P<0.001). Our results revealed that ACE Gene I/D polymorphism may be associated with osteoarthritis, ACE Gene polymorphism could be used as genetic markers in osteoarthritis in Han Chinese populations.
Disclosure: The authors declared no competing interests.