Background: The purpose of this study was to investigate the association between single nucleotide polymorphisms in neuropeptide genes and bone mineral density (BMD) in postmenopausal Korean women.
Methods: The 20 polymorphisms in the neuromedin (NMU) gene, NMU receptor 2 (NMU2R) gene, cocaine- and amphetamine-regulated transcript (CART) gene, neuropeptide Y (NPY) gene, NPY receptor 2 (NPY2R) gene, neuronal nitric oxide synthase (NOSI) gene, and melanocortin 4 receptor (MC4R) gene were analysed in 482 postmenopausal Korean women. Serum levels of bone turnover makers, leptin, soluble leptin receptor (sLR), osteoprotegerin (OPG), and soluble receptor activator of the nuclear factor-κB ligand (sRANKL) were measured and the BMD at the lumbar spine and femoral neck were also examined. The Institutional Review Board of our institution approved the study protocol.
Results: The CART rs2239670 was related to BMD of the lumbar spine, and the AG genotype had the highest BMD. Osteoporosis of the lumbar spine was more frequently observed in the GG genotype of the NPY rs17149106 and the CC genotype of the NPY rs16123 and less frequently observed in the TT-TT genotype identified by a combined polymorphism in the NPY2R gene, compared with the corresponding genotype. The AA genotype of the NOSI rs1279104 was found to have a 3.68-times higher frequency of osteoporosis at the femoral neck than the GG genotype. The adjusted serum levels of bone turnover markers, leptin, sLR, FLI, OPG, sRANKL, or sRANKL×1,000/OPG were not associated with the single polymorphisms measured in neuropeptide genes.
Conclusion: Our results suggest that the CART rs2239670 may be one of the genetic factors affecting lumbar spine BMD in postmenopausal Korean women, and that the NPY rs17149106, and rs16123, NOSI rs1279104, and combined polymorphism (rs2880415, rs6857715) in the NPY2R gene may be useful in identifying women at risk of osteoporosis.
Disclosure: The authors declared no competing interests. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0022334), and by grant from the Seoul National University College of Medicine Research fund (04-2013-0320).