Background: The aim was to investigate structure of the proximal epiphyseal cartilage (EC) of humerus after 60-day tartrazine intake and to find possibility of correction of the state with mexidol.
Methods: The study involved 175 male rats with body weight of 200-210 grams. The first group comprised the intact animals (C group), the second and the third groups comprised the animals that received per os tartrazine in dosage of 750 and 1500 mg per kg of body weight for 60 days (T1 and T2), and the fourth and the fifth groups received IM tartrazine and mexidol in dosage of 50 mg per kg of body weight (T1M and T2M). Upon expiration of observation terms (the 3rd, the 10th, the 15th, the 24th, and the 45th day) the frontal sections of HE stained proximal epiphyses were put to morphometry of zones of EC using classification of V.G. Koveshnikov (2003).
Results: By the third day of observation in the samples taken from T1, width of EC was lower than that of controls by 8.59%, width of osteogenic zone was respectively lower by 9.92%, amount of primary spongiosa and quantity of cells on trabecules surface were lower by 8.45% and 8.42%. In T2, the same values were lower by 11.52%, 11.91%, 9.84% and 9.49% as compared with the controls. From the 10th up to the 24th day, width of osteogenic zone for T1 was lower than that of C by 8.79%, 5.17%, and 5.32% and for T2 by 9.67%, 7.11%, and 5.33%. In the same period, osteoblast count in osteogenic zone for T1 was lower than that of the controls by 10.52%, 7.62%, and 5.83% and or T2 those values were lower by 11.31%, 8.10%, and 6.42%, respectively. Total width of EC in both groups by the 45th day was lower in comparison with the controls. Administration of mexidol together with tartrazine resulted in structure optimisation of the EC as compared with T1 and T2. Values characteristic of bone formation activities of the EC in T1M were higher than in T1 in the period from the 3rd up to the 45th day of observation and in T2M from the 15ht up to the 45th day of observation.
Conclusions: 60-day tartrazine intake results in inhibition of activities of EC of humerus. Degree and restoration rates directly depend on tartrazine dosage. Administration of mexidol in dosage of 50 mg per kg of body weight had a positive effect on restoration of epiphyseal cartilage of humerus in comparison with T1 and T2 groups.
Disclosure: The authors declared no competing interests.