ECTS Abstracts (2015) 1 P206

Effects of platelet-released supernatants with and without serum on differentiation and activity of osteoclasts and osteoblasts

Hermann Agis1,2, Stefan Schröckmair2,3, Carmine Skorianz2,3, Eva-Maria Mozgan2,3, Michael Edelmayer1,2, Michael Fischer4,5 & Reinhard Gruber3,6

1Department of Conservative Dentistry and Periodontology, Medical University of Vienna, Vienna, Austria; 2Austrian Cluster for Tissue Regeneration, Vienna, Austria; 3Department of Oral Surgery, Medical University of Vienna, Vienna, Austria; 4Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna, Austria; 5Center for Biomedical Technology, Danube University Krems, Krems, Austria; 6Laboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, Bern, Switzerland.

Platelet preparations are clinically applied to stimulate healing of oral tissue in regenerative dentistry. Platelets can modulate differentiation and activity of osteoclasts and osteoblasts. Research with different preparations of platelets is not conclusive. In the present study, we assessed if serum components modulate the effect of platelet preparations. In addition, we evaluated if collagen barrier membranes, which are clinically used in guided bone regeneration, can serve as carrier for platelet preparations. In murine bone marrow cultures, osteoclastogenesis was investigated in the presence of platelet-released supernatant (PRS) and serum containing PRS (SC-PRS). We quantified differentiation of osteoclasts based on the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNC), TRAP activity and resorption activity. In addition, viability and proliferation were tested. Osteoblastogenesis was assessed based on staining for alkaline phosphatase (AP). To assess mitogenic effects of collagen barrier membranes loaded with PRS and SC-PRS bioassays were performed and the release of platelet-derived growth factor (PDGF)-BB was measured. Our results show that PRS increases the number of TRAP positive MNC and their activity. SC-PRS decreased the number and activity of TRAP positive MNC. SC-PRS decreased formazan formation and 3[H]thymidine incorporation of osteoclast progenitors. Our results on osteoblastogenesis indicate that PRS can decrease the number of AP-positive colonies while SC-PRS can increased osteoblast markers. Proliferation of osteoblast-like cells was stimulated by all preparations. Collagen barrier membranes loaded with PRS and SC-PRS released PDGF-BB and stimulated proliferation. In conclusion, activated platelets stimulate differentiation of osteoclasts, while serum containing preparations decrease differentiation of osteoclasts and increase differentiation of osteoblasts. Our findings suggest that serum components modulate the effects of platelet preparations on osteoclastogenesis and osteoblastogenesis. Future studies will reveal the impact of these preparations on guided bone regeneration.

Disclosure: The authors declared no competing interests.

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