There has been a strong interest in searching for natural therapeutics for osteoporosis since natural medicines have fewer side effects and are more suitable for long-term use than synthesised drugs. This study aims to investigate the effects of Noni on osteogenic differentiation. Noni water extract was prepared. C2C12, mouse mesenchymal stem cells (mMSC) and human periodontal ligament cells (hPDL) were used to examine the effect of Noni. In the concentration range that did not show cytotoxicity, Noni significantly increased the expression levels of osteoblast differentiation marker genes, including alkaline phosphatase, Runx2, osterix and osteocalcin, in all of the cell types used in this study. In addition, Noni enhanced matrix mineralisation of mMSC and hPDL cells. Noni increased expression levels of BMP2, Wnt3a and beta-catenin. Furthermore, Noni stimulated TOP-Flash activity which was attenuated by addition of Dkk-1, a Wnt inhibitor. These results suggest that Noni extract promotes osteogenic differentiation of mesenchymal stem cells via enhancing the expression of Wnt ligands and subsequent activation of canonical Wnt/beta-catenin signalling.
Disclosure: The authors declared no competing interests. This research was supported by NRF Grant of Korea (2013R1A1A2058250, 2013).