The aim of this study was comparison of morphological, densitometric and mechanical properties of femur, tibia and tarsometatarsus in 14-month-old female ostriches (N=13). Bone length, bone weight and relative bone weight (RBW) were determined. Using quantitative computed tomography technique, cortical bone mineral density (Cd), mean volumetric bone mineral density (MvBMD), total bone volume (Bvol) and calcium hydroxyapatite density (Ca-HA) in the cortical bone were measured. Bone mineral density (BMD) and bone mineral content (BMC) were evaluated using dual-energy X-ray absorptiometry. Cortical bone area (CBA), cross-sectional area (A), second moment of inertia (Ix), mean relative wall thickness (MRWT) and cortical index (CI) were derived from the measurements of horizontal and vertical diameters of the investigated bones in the midshaft. Using three-point bending test, mechanical parameters of the bones (maximum elastic strength (Wy) and ultimate strength (Wf)) were determined. Bone weight, RBW, Bvol, BMC and Ca-HA of tibia were significantly higher when compared with these parameters in femur and tarsometatarsus (P≤0.01). Bone length and BMD reached the highest values in tibia than in tarsometatarsus and femur (both P≤0.01). MvBMD was the highest in tarsometatarsus than in tibia and femur (all P<0.001). Ix reached the highest value in femur than in tibia and tarsometatarsus (all P≤0.01). Cd, CBA, A and Wy were significantly lower in tarsometatarsus than in femur and tibia (P<0.05). MRWT and CI were significantly lower in femur when compared with tibia and tarsometatarsus (P<0.001). Wf of tarsometatarsus was significantly lower than in tibia (P=0.01). The obtained results have shown significant differences between morphological, densitometric and mechanical properties of the evaluated bones in female ostriches. Determination of morphological, densitometric and mechanical properties of femur, tibia and tarsometatarsus in female ostriches may serve for further studies on metabolic regulation of skeletal system properties with environmental, physiological, dietary, pharmacological and toxicological factors.
Disclosure: The authors declared no competing interests.