ECTS Abstracts (2015) 1 P310

Effect of Calcium Intake on the Interrelationship between Bone Turnover and Energetic Metabolism in Genetically Predisposed Obese Rats

Clarisa Marotte1, Hernandez Evelyn1,3, Weisstaub Adriana2, Somoza Julia1, Carlos Lugones1,3, Maria Luz de Portela2 & Susana N Zeni1,3

1INIGEM (Conicet-Uba), Buenos Aires, Argentina; 2Pharmacy and Biochemistry University (UBA), Buenos Aires, Argentina; 3Dentistry University UBA, Buenos Aires, Argentina.

Background: According to literature, bone, fat mass and pancreas are interrelated through osteocalcin (BGP), bone resorption, leptin and insulin levels. Ca intake might affect these interrelationships by inducing changes in bone resorption. Obesity could also affect bone and pancreas interrelationship through leptin effect on the osteoblastic insulin receptor or in sequestering vitamin D in fat pad. The present report evaluated changes in these interrelationship by feeding a low, normal or high Ca diet (LCa NCa and HCa, respectively), in growing genetically predisposed obese IIMb/( (O) rats.

Methods: Female O adult rats were mated and fed during pregnancy and lactation diets prepared according to AIN′93 recommendations that only varied in Ca content (0.2%, 0.5% and 0.9%). At weanling, male pups continued feeding the same diet ad libitum until 50 days of age. Food consumption and body weight (BW) were recorded 3 times/week. Serum Ca, phosphorus (P), BGP, CTX, 25OHD, glucose and insulin, and total body fat and adipose perigonadal and retroperitoneal percentage (PG+RP %) pads were determined.

Results: Results (mean±SE) are shown in Table 1.

Olca (N=10)ONCa (n=11)OHCa (n=10)
sCa (mg/dl)10.4±0.210.3±0.310.6±0.3
sP (mg/dl)11.9±0.710.5±0.6*10.9±0.4*
BGP (ug/ml)514±60375±46*739±191*,**
SCTX (mg/ml)70±483±7*69±13**
Insulin (mg/dl)6.9±1.24.1±0.8*1.9±1.3*,**
Glucose (mg/dl)252±49152±69*112±62*,**
25OHD (ng/mL)24.8±2.419.0±2.7*20.5±1.1*
Body Fat (g/100 BW)15.9±1.513.1±2.2*12.6±2.2*,**
(* and **): p<0.05 vs. OLCa and ONCa, respectively (ANOVA-Bonferroni). No significant differences were observed in sCa among the three O groups. OLCa presented the significantly highest P, insulin and glucose levels. CTX levels were lower in OLCa and OHCa than in ONCa. BGP levels increased as follow: OHCa >OLCa> ONCa. Fat and PG+RP% were inversely related to dietary Ca content.

Conclusion: The results suggested that both, Ca intake and dietary Ca/P ratio influence bone, fat mass and pancreas interrelationship in genetically predisposed obese IIMb/( rats. UBACyT 20020100100320/2011.

Disclosure: The authors declared no competing interests.

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